INST Faculty Directory

Dr. Surajit Karmakar

Associate Professor (Scientist E)

Educational background

    1997: BSc from University of Calcutta, India Subject: Physiology (Honors), Zoology, Chemistry
    1999: MSc from University of Calcutta, India Subject: Physiology
    2003: PhD from University of Calcutta, India Subject: Physiology

Positions held

♦ Post Doctoral Fellow at The Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, St Lucia, QLD 4072, Australia. 2012-2014
♦ Research Associate at Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.  2010-2011
♦ Postdoctoral fellow at Department of Pathology, Microbiology and Immunology and Chemical Engineering, University of South Carolina, Columbia, SC 29209, USA. 2008-2010
♦ Postdoctoral fellow at Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA. Employment Period: 2004-2007

Research Interest

Development of cancer nano-therapeutics including screening of peptide and small molecule combinatorial therapy, siRNA delivery and peptide-mediated cancer therapy by targeting mitochondrial metabolism. Understanding molecular mechanisms to overcome resistance to chemotherapy, target validation, signal transduction in membrane proteins and nanoparticles endocytotic cascade. Receptor and ion channel regulation on cell membrane organization, endosomal function and escape.

Awards

♦ New Staff Development Grant: University of Queensland, 2013
♦ Young Investigator Award, American Society for Neurochemistry, 2005
♦ Senior Research Fellow, ICMR, New Delhi, India, 2002-2004

Selected Publications

♦ Yu M,Karmakar S, Yang J, Zhang H,Thorn P, Yu C (2014) Facile synthesis of ultra-small hybrid silica spheres for enhanced penetration of 3D glioma spheroids. ChemComm. 50:1527-9.
♦ Yang Y, Karmakar S, Yu M, Popat A, Yu C (2014) Synthesis of Silica Vesicles with Small Sizes and Reduced Aggregation for Photodynamic Therapy. Chemistry Letters Vol. 43 (2014) No. 3 P 316-318.
♦ Xu C, Niu Y, Popat A, Jambhrunkar S, Karmakar S, Yu C (2014) Rod-like Mesoporous Silica Nanoparticles with Rough Surfaces for Enhanced Cellular Delivery. Journal of Materials Chemistry B. 2 3: 253-256.
♦ Popat A, Karmakar S, Jambhrunkar S, Xu C, Yu C (2014) Curcumin-cyclodextrin Encapsulated Chitosan Nanoconjugates with Enhanced Solubility and Cell Cytotoxicity. Colloids and Surfaces B: Biointerfaces (Accepted, In Press).
♦ Jambhrunkar S, Karmakar S,Popat A,Yu M, Yu C (2014) Mesoporous Silica Nanoparticles Enhance the Cytotoxicity of Curcumin.  RSC Advances 4 2: 709-712.
♦ Niu Y, Popat A,  Yu M, Karmakar S, Gu W, Yu C (2012) Recent advances in the rational design of silica-based nanoparticles for gene therapy. Therapeutic Delivery, 3, 1217-37. PMID: 23116013.
♦ Choudhury SR, Karmakar S, Banik NL, Ray SK (2012) Targeting Angiogenesis for Controlling Neuroblastoma. J Oncol. 2012:782020. PMID: 21876694.
♦ Mohan N, Karmakar S, Banik NL, Ray SK (2011) SU5416 and EGCG Work Synergistically and Inhibit Angiogenic and Survival Factors and Induce Cell Cycle Arrest to Promote Apoptosis in Human Malignant Neuroblastoma SH-SY5Y and SK-N-BE2 Cells. Neurochem Res 36:1383-96. PMID: 21472456.
♦ Karmakar S, Choudhury SR, Banik NL, Ray SK (2011) N-(4-Hydroxyphenyl) Retinamide Potentiated Anti-Tumor Efficacy of Genistein in Human Ewing’s Sarcoma Xenografts. World J Oncol 2:53-63. PMID: 21822457.
♦ Karmakar S, Choudhury SR, Banik NL, Ray SK (2011) Induction of Mitochondrial Pathways and Endoplasmic Reticulum Stress for Increasing Apoptosis in Ectopic and Orthotopic Neuroblastoma Xenografts. J Cancer Therapy, 2:77-90. 
♦ Karmakar S, Choudhury SR, Banik NL and Ray SK (2011) Molecular Mechanisms of the Anti-cancer Action of Garlic Compounds in Neuroblastoma cells. Anti-Cancer Agents in Medicinal Chemistry. 11:398-407. PMID: 21521157.
♦ Choudhury SR, Karmakar S, Banik NL, Ray SK (2011) Valproic Acid Induced Differentiation and Potentiated Efficacy of Taxol and Nanotaxol for Controlling Growth of Human Glioblastoma LN18 and T98G Cells. Neurochem Res 36:2292-305. PMID: 21786169.
♦ Choudhury SR, Karmakar S, Banik NL, Ray SK (2010) Downregulation of angiogenic and survival factors increased apoptosis through upregulation of p53 and p21 in malignant neuroblastoma cells having N-Myc amplification or non-amplification. Investigational New Drugs 28:812-24. PMID: 19777160.
♦ Karmakar S, Choudhury SR, Banik NL, Ray SK (2010)  Activation of Multiple Molecular Mechanisms for Increasing Apoptosis in Human Glioblastoma T98G Xenograft. J Cancer Sci Ther 2: 107-113. PMID: 21666767
♦ Mohan N, Karmakar S, Choudhury SR, Banik NL, Ray SK (2009) Bcl-2 inhibitor HA14-1 and genistein together adeptly down regulated survival factors and activated cysteine proteases for apoptosis in human malignant neuroblastoma SK-N-BE2 and SH-SY5Y cells. Brain Res 1283:155-166. PMID: 19505441.
♦ Karmakar S, Davis KA, Choudhury SR, Deeconda A, Banik NL, Ray SK (2009) Bcl-2 inhibitor and apigenin worked synergistically in human malignant neuroblastoma cell lines and increased apoptosis with activation of extrinsic and intrinsic pathways. BBRC 388:705-710. PMID: 19695221.
♦ Karmakar S, Choudhury SR, Banik NL, Ray SK (2009) Combination of N-(4-hydroxyphenyl) retinamide and genistein increased apoptosis in neuroblastoma SK-N-BE2 and SH-SY5Y xenografts. Neuroscience 163:286-295. PMID: 19540315.
♦ Karmakar S, Banik NL, Ray SK  (2008) Combination of all-trans retinoic acid and paclitaxel-induced differentiation and apoptosis in human glioblastoma U87MG xenografts in nude mice. Cancer 112: 596-607. PMID: 18098270.
♦ Karmakar S, Foster Olive M, Banik NL, Ray SK (2007)  Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor. Neurochem Res 32:2235-2242. PMID: 17701349.
♦ Karmakar S, Banik NL, Ray SK  (2007) Molecular Mechanism of Inositol Hexaphosphate-mediated Apoptosis in Human Malignant Glioblastoma T98G Cells. Neurochem Res 32:2094-2102. PMID: 17616815.
♦ Karmakar S, Banik NL, Ray SK   (2007) Curcumin Suppressed Anti-apoptotic Signals and Activated Cysteine Proteases for Apoptosis in Human Malignant Glioblastoma U87MG Cells. Neurochem Res 32:2103-2113. PMID: 17562168.
♦ Karmakar S, Banik NL, Patel SJ, Ray SK (2007) Combination of all-trans retinoic acid and taxol regressed glioblastoma T98G xenografts in nude mice. Apoptosis 12:2077-2087. PMID: 17701358.
♦ Karmakar S, Banik NL, Patel SJ, and Ray SK (2007) Garlic compounds induce calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells.  Apoptosis 12:671-684. PMID: 17219050.
♦ Karmakar S, Banik NL, Patel SJ, and Ray SK (2007) 5-Aminolevulinic acid-based photodynamic therapy suppressed survival factors and activated proteases for apoptosis in human malignant glioblastoma U87MG cells.  Neurosci Lett 415:242-247. PMID: 17335970.
♦ Ray SK, Karmakar S, Nowak MW, and Banik NL (2006) Inhibition of calpain and caspase-3 prevented apoptosis and preserved electrophysiological properties of voltage-gated and ligand-gated ion channels in rat primary cortical neurons exposed to glutamate. Neuroscience 139: 577-595. PMID: 16504408.
♦ Karmakar S, Weinberg M, Banik NL, Patel SJ, and Ray SK (2006) Activation of multiple molecular mechanisms for apoptosis in human malignant glioblastoma cell lines T98G and U87MG treated with sulforaphane. Neuroscience 141: 1265-1280. PMID: 16765523.
♦ Karmakar S, Banik NL, Patel SJ, and Ray SK (2006) Curcumin activated both receptor-mediated and mitochondria-mediated proteolytic pathways for apoptosis in human glioblastoma T98G cells. Neurosci Lett 407: 53-58. PMID: 16949208.
♦ Karmakar S, Muhuri DC, Dasgupta SC, Nagchaudhuri AK, Gomes A (2004) Isolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract. Ind J Exp Biol 42:452-460. PMID: 15233468.
♦ Muhuri D, Karmakar S, Dasgupta SC, Nagchaudhuri AK, Gomes A (2004)  Pharmacological studies on the venomous spotted butterfish (Scatophagus argus Linn) sting extract on experimental animals. Ind J Exp Biol 42:461-467. PMID: 15233469.
 ♦ Karmakar S, Das T, Ghosh A, Dasgupta SC, Biswas AK, Gomes A (2004) Isolation and partial structural evaluation of a cardiotoxic factor from Indian common murrel (Channa striatus L.) skin extract. Ind J Exp Biol 42:271-278. PMID: 15233296.
♦ Karmakar S, Dasgupta SC, Gomes A (2002) Pharmacological and haematological study of shol fish (Channa striatus) skin extract on experimental animal. Ind J Exp Biol 40:115–118. PMID: 12561982.

BOOK CHAPTER PUBLICATIONS
♦ Karmakar S, Banik NL, Ray SK (2010) Current endeavors for enhancing efficacy of paclitaxel for treatment of glioblastoma. In Glioblastoma: Molecular Mechanisms of Pathogenesis and Current Therapeutic Strategies. Publisher: Springer; 1 edition, Page: 299-323, ISBN-978-1-4419-0409-6.

♦ Roy Choudhury S, Karmakar S, Banik NL, Ray SK (2010) Role of angiogenesis in the pathogenesis of glioblastoma and antiangiogenic therapies for controlling glioblastoma. In Glioblastoma: Molecular Mechanisms of Pathogenesis and Current Therapeutic Strategies. Publisher: Springer; 1 edition, Page: 217-241, ISBN-978-1-4419-0409-6.

Conferences


♦ Davis KA, Karmakar S, Choudhury SR, Banik NL and Ray SK (2010) Synergistic efficacy of HA14-1 and apigenin in inhibiting angiogenic factors and increasing apoptosis in neuroblastoma cells. Frontiers in Neuroscience Conference. March 05 - 07, 2010, Atlanta, USA.
♦ Karmakar S, Banik NL, Ray SK (2009)Combination of ATRA and IFN-γ for increasing apoptosis in glioblastoma U87MG xenografts in nude mice. Annual ASN Meeting, Charleston, SC - March 7-11 2009, USA.
♦ Roy Choudhury S, Karmakar S, Banik NL, Ray SK (2009) Combination of valproic acid and taxol or nanotaxol induced differentiation and apoptosis in glioblastoma LN18 and T98G cells. Annual ASN Meeting, Charleston, SC - March 7-11 2009, USA.
♦ Mohan N, Karmakar S, Roy Choudhury S, Banik NL , Ray SK (2009) Small Molecule Inhibitor of Bcl-2 Enhances Efficacy of Genistein for Apoptosis in Neuroblastoma SH-SY5Y and SK-N-BE2 Cell lines. Annual ASN Meeting, Charleston, SC - March 7-11, 2009, USA.
♦ Davis K, Karmakar S, Roy Choudhury S, Deeconda A, Banik NL, Ray SK (2009)  Combination of Bcl-2 inhibitor and apigenin activated extrinsic and intrinsic pathways of apoptosis in neuroblastoma SK-N-DZ cells. Annual ASN Meeting, Charleston, SC - March 7-11, 2009, USA.
♦ Roy Choudhury S, Karmakar S, Banik NL, Ray SK (2009) Combination of sorafenib and genistein inhibits angiogenesis and triggers multiple molecular mechanisms for apoptosis in human malignant neuroblastoma SK-N-DZ and SH-SY5Y cell lines. AACR Annual Meeting April 18-22, 2009, Denver, CO, USA.
♦ Karmakar S, Roy Choudhury S, Tomlinson S, Banik NL, Ray SK (2009)Combination of N-(4-hydroxyphenyl) retinamide and genistein increases apoptosis in Ewing’s sarcoma SK-N-MC cells in culture and xenografts in athymic nude mice. AACR Annual Meeting April 18-22, 2009, Denver, CO, USA.
♦ Karmakar S, Banik NL, Ray SK (2008)Development of guide-cannula intracranial allograft model of glioblastoma in Sprague-Dawley rats.  Annual ASN Meeting, San Antonio, Texas, March 1 - 5, 2008, USA.
♦ Karmakar S, Walle T, Banik NL, Ray SK  (2008)  Treatment with combination of all-trans retinoic acid and interferon-gamma regressed human glioblastoma T98G xenografts in nude mice. Annual meeting of the American Association for Cancer Research (AACR), April 14-18, San Diego, CA, USA.
♦ Karmakar S, Patel SJ, Banik NL, Ray SK (2007)Photodynamic therapy activated proteolytic pathways for apoptosis in human glioblastoma U87MG cells. Frontier Research Day, MUSC, SC, USA.
♦ Karmakar S, Patel SJ, Walle T, Banik NL, Ray SK (2007) Combination of N-(4-hydroxyphenyl) retinamide and genistein induced differentiation and multiple molecular mechanisms for apoptosis in human malignant neuroblastoma SK-N-BE(2) xenografts in nude mice.  Annual meeting of the American Association for Cancer Research (AACR), April 14-18, Los Angeles, CA, USA.
♦ Karmakar S, Banik NL, Patel SJ, and Ray SK (2006) Curcumin activated calpain and caspases in human malignant glioblastoma U87MG cells for apoptosis. AACR Annual Meeting, April 1-5, 2006 at Washington, DC.
♦ Karmakar S, Banik NL, Patel SJ, and Ray SK (2006)  Molecular mechanism of inositol hexaphosphate mediated apoptosis in human malignant glioblastoma T98G cells.  Program No. 389.9. 2006 Abstract Viewer/Itinerary Planner. Atlanta, GA: Society for Neuroscience, USA.
♦ Mao T, Karmakar S, Banik NL, Ray SK (2006) Inositol hexaphosphate induced apoptosis in human malignant glioblastoma U87MG cells. MUSC Student Research Day. Charleston, SC, USA.
♦ Karmakar S, Das A, Banik NL, Patel SJ, Ray SK (2005)   Induction of calpain and intrinsic caspase  pathways for apoptosis in human malignant neuroblastoma cell line SHSY-5Y following exposure to dially sulphide (DAS) and diallyl disulphide (DADS). AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Karmakar S, Weinberg MS, Das A, Banik NL, Patel SJ, Ray SK (2005) Activation of multiple molecular mechanisms for of apoptosis in human malignant glioblastoma cell lines T98G and U87MG following exposure to L-sulphoraphane. AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Karmakar S, Das A, Tomlinson S, Banik NL, Patel SJ, Ray SK (2005) Treatment with combination of all trans-retinoic acid and taxol induced differentiation and apoptosis in human malignant glioblastoma U87MG cells xenografted in athymic nude mice. AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Karmakar S, Das A, Banik NL, Patel SJ, Ray SK (2005)Combination of retinoid and flavonoid controlled the growth of human malignant neuroblastoma SHSY5Y cells in culture as well as xenografted athymic nude mice.  AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Das A, Karmakar S, Patel SJ, Banik NL, and  Ray SK (2005) All- trans retinoic acid and 13-cis retinoic acid differentiated rat C6 glioblastoma cells and decreased telomerase activity leading to increased sensitivity to IFN- γ and taxol for apoptosis. AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Das A, Karmakar S, Patel SJ, Banik NL, and Ray SK (2005)Activation of proteolytic activities of calpain and caspase-3 in apoptosis of human malignant glioblastoma cell lines T98G and U87MG exposed to diallyl sulphide (DAS) and dially disulphide (DADS). AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Das A, Karmakar S, Styart RK, Patel SJ, Banik NL, and Ray SK (2005)Plant derived flavonoids induced proteolytic activities of calpain and caspase-3 for apoptosis in human malignant glioblastoma U87MG cells. AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Das A, Karmakar S, Patel SJ, Priest DG, Banik NL, and Ray SK(2005) Differentiation of human glioblastoma U87MG cells with all-trans retinoic acid (ATRA) or 13-cis retinoic acid (13-CRA) down regulated telomerase activity and enhanced sensitivity to interferon –gamma (IFN-γ) or taxol (TXL) for apoptosis. AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Das A, Karmakar S, Patel SJ, Banik NL, and Ray SK(2005) Exploration of the potential of N-(4-hydroxyphenyl) retinamide (4-HPR) for induction of both differentiation and apoptosis in two human malignant glioblastoma cell lines T98G and U87MG. AACR Annual Meeting April 16 - 20, 2005, Anaheim, California.
♦ Ray SK, Das A, Karmakar S, Banik NL, Patel SJ (2005) Activation of proteolytic pathways by polyphenolic phytochemicals for apoptosis in human glioblastoma T98G cells. European Winter Oncology Conference, Sunday 16 - Friday 21 May 2005 at  Edinburgh, United Kingdom.
♦ Ray SK, Karmakar S, Das A, Banik NL, Patel SJ (2005) Human glioblastoma T98G cells xenografted in athymic nude mice overwhelmingly committed after treatment with all-trans retinoic acid and taxol. European Winter Oncology Conference, Sunday 16 - Friday 21 May 2005 at  Edinburgh, United Kingdom.
♦ Karmakar S, Saha A, Das A, Banik NL, Ray SK (2005)  Curcumin activated multiple molecular mechanisms for   apoptosis in human glioblastoma T98 cells. Annual Meeting of American Society for Neurochemistry at Madison, Wisconsin, June 25-29, 2005.
♦ Das A, Karmakar S, Saha A, Banik NL, Ray SK (2005) Dexamethasone decreases while acetazolomide increases   temozolomide induced apoptosis in human glioblastoma cells. Annual Meeting of American Society for Neurochemistry at Madison, Wisconsin, June 25-29, 2005.
♦ Karmakar S, Banik NL, Patel SJ, and Ray SK (2005)  A combination therapy for enhancement of apoptosis in human malignant glioblastoma T98G and U87MG cells xenografted in nude mice. International Symposium on Translational Research: Apoptosis and Cancer.
♦ Fry P, Karmakar S, Matzelle D, Banik NL, Ray SK (2005) A potential role for calpain in pathogenesis of kainic acid-induced epileptic seizures in sprague-dawley rats. MUSC Student Research Day., SC, USA.
♦ Karmakar S, Yallapragada AV, Das A, Banik NL, Patel SJ, Ray SK  (2004) Activation of proteolytic mechanisms in apoptosis of human malignant neuroblastoma SH-SY5Y cells exposed to diallyl disulphide (DADS). MUSC Student Research Day.
♦ Das A, Banik NL, Karmakar S, Patel SJ, Ray SK (2004) All-trans retinoic acid decreased telomerase activity and increased IFN-γ and taxol induced apoptosis in human glioblastoma T98G cells. SFN Annual Meeting.
♦ Karmakar S, Gomes A (2003) Occurrence of an antineoplastic compound (CSS-ANF) from the skin of the snake head fish Channa striatus, Linn. XXXVI Annual Conference of the Indian Pharmacological Society.

Patent

A process for the isolation of a novel antineoplastic compound useful for pharmacological purposes. Gomes A, Karmakar S, Gomes A, Das T, Ghosh A, Biswas AK, Dasgupta SC (Patent filed through the Intellectual Property Cell, ICMR, New Delhi; Ref. No: 832/Del/2003, June 23, 2003).

Office Address:

Institute of Nano Science & Technology
Habitat Centre, Phase X, Sector - 64, Mohali, Punjab-160062, India

Contact info:

Tel: 0172 - 2210075 (Ext. 1116
Email Id : surajit(at)inst(dot)ac(dot)in