INST Faculty Directory
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Dr.Sharmistha Sinha

Assistant Professor (Scientist D)
Educational and Other Qualification:

Education: 2006 PhD (Biophysics), Molecular Biophysics Unit, Indian Institute of Science, Bangalore, INDIA.

Awards and Distinction:

♦ Recipient of the INSA Medal for Young Scientist 2008, Indian National Science Academy, Government of India.

Positions held:

♦ Postdoctoral Fellow, Dept. of Biochem. Biophys. and Mol. Biol., Iowa State University, Ames, USA from 2010-2013
♦ Postdoctoral  Fellow, Dept. of Neurology, University of California, Los Angeles, USA from 2008-2010
♦ Research Associate, Indian Institute of Science, Bangalore, India from 2007-2008.

Research interests

♦ Biomaterials based on Prokaryotic Protein Assemblies
♦ Ligand-Protein interactions
♦ Protein Structure-Function relationship
♦ Genetic engineering of prokaryotic macromolecular assemblies

Recent Publications

1.  S Sinha, S Cheng, YW Sung, DE McNamara, MR Sawaya, TO Yeates, TA Bobik. Alanine scanning mutagenesis identifies an asparagine-arginine-lysine triad essential to assembly of the shell of the pdu microcompartment. J Mol Biol. 2014;426(12):2328-45.
2. S. Cheng, Fan, C.,S Sinha, T A Bobik The PduQ enzyme is an alcohol dehydrogenase used to recycle NAD+ internally within the Pdumicrocompartment of Salmonella enterica. PlosOne20127(10):e47144.
3. Fan, C.,S. Cheng, S Sinha, T A Bobik.  Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments.Proc. Natl. Acad. Sc. USA2012109(37):14995-5000.
4. Sinha, S., Lopes, DHJ. And Bitan G, A key role for lysine residues in amyloid beta-protein folding, assembly, and toxicity. ACS Chemical Neuroscience.2012.3 (6): 473–481.
5. S. Sinha, Z. Du, P. Maiti, F.-G. Klärner, T. Schrader, C. Wang, and G. Bitan, Comparison of three amyloid assembly inhibitors – the sugar scyllo-inositol, the polyphenol epigallocatechingallate, and the molecular tweezers CLR01. ACS Chemical Neuroscience.2012 3 (6):451–458.
6. S. Prabhudesai,* S. Sinha*, A. Attar, A. Kotagiri, A.G. Fitzmaurice, R. Lakshmanan, M.I. Ivanova, J.A. Loo,  F-G Klärner, T. Schrader, M. Stahl, G. Bitan, JM Bronstein. A Novel “Molecular Tweezer” Inhibitor of α-Synuclein Neurotoxicity in vitro and in vivo.Neurotherapeutics.2011 9(2):464-76(* equal contribution)
7. Sinha, S, S. Cheng, C. Fan, T. Bobik. The PduM protein is a structural component of the microcompartments involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella. J. Bacteriol.194(8):1912-8.
8. Sinha, S., D.H. Lopes, Z. Du, E.S. Pang, A. Shanmugam, A. Lomakin, P. Talbiersky, A. Tennstaedt, K. McDaniel, R. Bakshi, P.Y. Kuo, M. Ehrmann, G.B. Benedek, J.A. Loo, F.G. Klarner, T. Schrader, C. Wang, and G. Bitan, Lysine-specific molecular tweezers are broad-spectrum inhibitors of assembly and toxicity of amyloid proteins. J. Am. Chem. Soc., 2011. 133(42): 16958-69.
9. Hochdorffer, K., J. Marz-Berberich, L. Nagel-Steger, M. Epple, W. Meyer-Zaika, A.H. Horn, H. Sticht, S. Sinha, G. Bitan, and T. Schrader, Rational design of beta-sheet ligands against Ab42-induced toxicity. J. Am. Chem. Soc., 2011. 133(12): 4348-58.
10. Cheng, S., S. Sinha, C. Fan, Y. Liu, and T.A. Bobik, Genetic analysis of the protein shell of the microcompartments involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella. J. Bacteriol., 2011. 193(6): p. 1385-92

11. S. Sinha, Z. Du, P. Maiti, F.-G. Klärner, T. Schrader, C. Wang, and G. Bitan, Comparison of three amyloid assembly inhibitors – the sugar scyllo-inositol, the polyphenol epigallocatechingallate, and the molecular tweezers CLR01. ACS Chemical Neuroscience.2012 3 (6):451–458.

12. S. Prabhudesai,* S. Sinha*, A. Attar, A. Kotagiri, A.G. Fitzmaurice, R. Lakshmanan, M.I. Ivanova, J.A. Loo,  F-G Klärner, T. Schrader, M. Stahl, G. Bitan, JM Bronstein. A Novel “Molecular Tweezer” Inhibitor of α-Synuclein Neurotoxicity in vitro and in vivo.Neurotherapeutics.2011 9(2):464-76(* equal contribution)

13. Surolia, I*., S. Sinha*, D.P. Sarkar, P.Y. Reddy, G.B. Reddy, and A. Surolia, Concurrence of Danish dementia and cataract: insights from the interactions of dementia associated peptides with eye lens alpha-crystallin. PLoS One, 2008. 3(8): p. e2927. (*equal contribution)

14. Sinha, S. and A. Surolia, Attributes of glycosylation in the establishment of the unfolding pathway of soybean agglutinin. Biophys. J., 2007. 92(1): p. 208-16.

15. Sinha, S., G. Gupta, M. Vijayan, and A. Surolia, Subunit assembly of plant lectins. Curr. Opin. Struct. Biol., 2007. 17(5): p. 498-505.

16. Sinha, S. and A. Surolia, Oligomerization endows enormous stability to soybean agglutinin: a comparison of the stability of monomer and tetramer of soybean agglutinin. Biophys. J., 2005. 88(6): p. 4243-51.

17. Sinha, S., N. Mitra, G. Kumar, K. Bajaj, and A. Surolia, Unfolding studies on soybean agglutinin and concanavalin a tetramers: a comparative account. Biophys. J., 2005. 88(2): p. 1300-10.

Book Chapters:

♦ Amyloids and Protein Aggregation—Analytical Methods.   Li H, F Rahimi, K Murakami, SSinha, P Maiti, G Bitan. Encyclopedia of Analytical Chemistry. 2009
♦ Application of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins. Lopes DHJ, S Sinha, C Rosensweig, G Bitan. Methods Mol Biol. 849:11-21.2012

Patents :

Sinha,S., P. Talbiersky, A. Lomakin, F.-G. Klärner, T. Schrader, S. Frautschy, and G. Bitan. International Patent Application No. PCT/US2010/026419, titled "Molecular Tweezers for the Treatment of Amyloid-Related Diseases". (Pending)
♦ T Schrader, K Hochdorffer, J Marz-Berberich, L Nagel-Steger, G Bitan, S Sinha. International Patent Application No. PCT/EP2010/000437, titled “New compounds for the treatment of diseases related to protein misfolding”

Conferences/Talks :

♦ The 56th Biophysical Society Meeting held at SanDiego, CA 2012.
♦ The 23rd Symposium of the Protein Society, Boston, MA 2009.
♦ XVIII International Symposium on Glycoconjugates (GLYCO-18), Florence, Italy, 2005.
♦ XVII International Symposium on Glycoconjugates (GLYCO-17), Bangalore, 2002

CONTACT INFORMATION :

Tel: 0172 - 2210075
Email Id : sinhas(at)inst(dot)ac(dot)in

Office Address :

Institute of Nano Science & Technology
Habitat Centre, Sector - 64, Phase X, Mohali