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(Associate Professor, Scientist-E, Head of the Unit)
Variable Mutations at the p53-R273 Oncogenic Hotspot Position Leads to Altered Properties
This work stems from an interesting observation that genetic mutations result in the switching of one amino acid to different variants at the same codon show different cancer pathogenesis. We are trying to comprehend the molecular mechanism for the variation in gain-of-function opted by these variants. Several complementary experiments aided with computational studies show that residue-specific mechanism of unfolding and self-assembly of the entire protein might be one of the crucial parameters for their different oncogenic traits. These studies thus call for the need for developing therapeutic strategies that consider the resultant mutant-variant as a target rather than a mutation position. This is an important lead towards the understanding of the molecular contribution of p53 mutant aggregation in cancer.
(Biophys. J. Volume 118, Issue 3, 2020, Pages 720-728, https://doi.org/10.1016/j.bpj.2019.12.015 )
Email: smrose.ph21234@inst.ac.in
Reg. No.: PH21234
Email: aarcha.ph21202@inst.ac.in
Reg. No.: PH21202
Email: preeti.ph20209@inst.ac.in
Reg. No.: PH20209
Email: dimple.ph20210@inst.ac.in
Reg. No.: PH20210
Email: simerpreet.ph17224@inst.ac.in
Reg. No.: PH17224
Email: harpreet.ph19201@inst.ac.in
Reg. No.: PH19201
Email: gaurav.phd17202@inst.ac.in
Reg. No.: Ph17202
Email: silky.ph19202@inst.ac.in
Reg. No.: PH19202
Email: ankush@inst.ac.in
Reg. No.: PH15206
Email: harmanjit.ph14223@inst.ac.in
Reg. No.: ph14223
Email: barinaimat@gmail.com
Reg. No.: PH14210
Naturally occurring protein nano compartments: basic structure, function, and genetic engineering: Dimple Goel,Sharmistha Sinha, (2021) Nano Express, 2 (4): 042001. DOI: 10.1088/2632-959X/ac2c93/
Factors deciding the assembly and thermostability of the DmrB cage: Ankush Garg, (2021) International Journal of Biological Macromolecules 182, 959-967, .
Biophysical approaches to understand and re-purpose bacterial microcompartments: Gaurav Kumar,Sharmistha Sinha, (2021) Current Opinion in Microbiology, 63,: 43-51. DOI: 10.1016/j.mib.2021.05.008
Protein morphology drives the structure and catalytic activity of bio-inorganic hybrids: Harpreet Kaur,Naimat K Bari,Ankush Garg,Sharmistha Sinha, (2021) International Journal of Biological Macromolecules, Just Accepted: . DOI: 10.1016/j.ijbiomac.2021.01.217
Temporal control in tritylation reactions through light-driven variation in chloride ion binding catalysis–A proof of concept: Surbhi Grewal Saonli Roy Himanshu Kumar Mayank Saraswat Naimat K Bari Sharmistha Sinha Sugumar Venkataramani, (2020) Catalysis Science & Technology, 10 (20): 7027-7033. DOI: 10.1039/D0CY01090A
Probe into a Multi-Protein Prokaryotic Organelle Using Thermal Scanning Assay Reveals Distinct Properties of the Core and the Shell: Dr. Naimat K Bari ,Jagadish P Hazra ,Gaurav Kumar,Simerpreet Kaur,SHARMISTHA SINHA, (2020) BBA - General Subjects, 1864(10): 129680. DOI: 10.1016/j.bbagen.2020.129680
Variable Mutations at the p53-R273 Oncogenic Hotspot Position Leads to Altered Properties: Ankush Garg , Jagadish Prasad Hazra 2, Malay Kumar Sannigrahi 2, Sabyasachi Rakshit 3, Sharmistha Sinha, (2019) Biophysical Journal, 118 (3): 720-728. DOI: 10.1016/j.bpj.2019.12.015
Functional protein shells fabricated from the self-assembling protein sheets of prokaryotic organelles: Naimat K. Bari, Gaurav Kumar , Jagadish P. Hazra, Simerpreet Kaur ,Sharmistha Sinha, (2019) Journal of Materials Chemistry B, 8: 523-533. DOI: https://doi.org/10.1039/C9TB02224D
Cellulose-metallothionein matrix for metal binding: Naimat K.Bari ,Shaswat Barua ,Ankush Garg,Malay K Sannigrahi,Sharmistha Sinha, (2018) Carbohydrate polymers, 192: 126-134. DOI: 10.1016/j.carbpol.2018.03.043
Nanoparticle Fabrication on Bacterial Microcompartment Surface for the Development of Hybrid Enzyme-Inorganic Catalyst.: Naimat Kalim Bari , Gaurav Kumar, Aashish Bhatt, Jagadish Prasad Hazra, Ankush Garg, Md. Ehesan Ali, (2018) ACS Catal., 8: 7742–7748. DOI: 10.1021/acscatal.8b02322
The Wrappers of the 1,2-Propanediol Utilization Bacterial Microcompartments.: Naimat K Bari, Gaurav Kumar, Sharmistha Sinha, (2018) Adv Exp Med Biol ., 1112: 333-344.. DOI: 10.1007/978-981-13-3065-0_23
Enhanced bacterial cellulose production from Gluconobacter xylinus using super optimal broth: Prathna T. Chandrasekaran, Naimat Kalim Bari, (2017) Cellulose, 24(10): 4367–4381.
Selective molecular transport through the protein shell of a bacterial microcompartment organelle: Chowdhury C, Chun S, Pang A, Sawaya MR, Sinha S, Yeates TO, Bobik TA., (2015) Proc Natl Acad Sci U S A., 112(10): 2990-5. DOI: 10.1073/pnas.1423672112
Alanine scanning mutagenesis identifies an asparagine-arginine-lysine triad essential to assembly of the shell of the Pdu microcompartment: Shouqiang Cheng, Yea Won Sung, Dan E. McNamara, Michael R. Sawaya, Todd O. Yeates, Thomas A. Bobik. J., (2014) J. Mol. Biol., 26(12): 2328-45. DOI: 10.1016/j.jmb.2014.04.012
Diverse bacterial microcompartment organelles: Chowdhury C, Sinha S, Chun S, Yeates TO, Bobik TA., (2014) Microbiol Mol Biol Rev., 78(3): 438-68. DOI: 10.1128/MMBR.00009-14
Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments: Sharmistha Sinha, (2012) PNAS, 109(37): 14995-5000. DOI: doi: 10.1073/pnas.1207516109
Comparison of Three Amyloid Assembly Inhibitors: The Sugar Scyllo-Inositol, the Polyphenol Epigallocatechin Gallate, and the Molecular Tweezer CLR01: Sharmistha Sinha 1, Zhenming Du, Panchanan Maiti, Frank-Gerrit Klärner, Thomas Schrader, Chunyu Wang, Gal Bitan, (2012) ACS Chemical Neuroscience, 3(6): 451-8. DOI: DOI: 10.1021/cn200133x
A Key Role for Lysine Residues in Amyloid β-Protein Folding, Assembly, and Toxicity: Sharmistha Sinha,Dahabada H. J. Lopes,Gal Bitan. ACS Chem. Neurosci., (2012) ACS Chem. Neurosci., 3 (6): 473–481. DOI: 10.1021/cn3000247
Lysine-Specific Molecular Tweezers Are Broad-Spectrum Inhibitors of Assembly and Toxicity of Amyloid Proteins: Sharmistha Sinha, Dahabada H. J. Lopes, Zhenming Du, Eric S. Pang, Akila Shanmugam, Aleksey Lomakin, Peter Talbiersky, Annette Tennstaedt, Kirsten McDaniel, Reena Bakshi, Pei-Yi Kuo, Michael Ehrmann, George B. Benedek, Joseph A. Loo, Frank-Gerrit Klärner, Thomas Schrader, Chunyu Wang, and Gal Bitan J. Am. Chem. Soc, (2011) J. Am. Chem. Soc., 133 (42): 16958–16969. DOI: 10.1021/ja206279b
Application of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins: Lopes DHJ, C Rosensweig, G Bitan., (2012) Methods Mol Biol., 849: 11-21.
Amyloids and Protein Aggregation—Analytical Methods: Li H, F Rahimi, K Murakami, P Maiti, G Bitan., (2009) Encyclopedia of Analytical Chemistry., .
Molecular Tweezers for the Treatment of Amyloid-Related Diseases: P. Talbiersky, A. Lomakin, F.-G. Klärner, T. Schrader, S. Frautschy,G. Bitan., (2010) .
New compounds for the treatment of diseases related to protein misfolding: T Schrader, K Hochdorffer, J Marz-Berberich, L Nagel-Steger, G Bitan, (2010) .
XVIII International Symposium on Glycoconjugates (GLYCO-18): Florence, Italy, (2005) .
The 56th Biophysical Society Meeting: SanDiego, CA, (2012) .
The 23rd Symposium of the Protein Society, Boston: MA, (2009) .
XVII International Symposium on Glycoconjugates (GLYCO-17): Bangalore, (2002) .
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, INDIA.
Postdoctoral Fellow:Dept. of Biochem. Biophys. and Mol. Biol., Iowa State University, Ames,, USA (April 2010 to April 2013 )
Postdoctoral Fellow:Dept. of Neurology, University of California, Los Angeles,, USA (March 2008 to March 2010 )
Research Associate:Indian Institute of Science, Bangalore,, India (April 2007 to April 2008 )
Recipient of the Har Govind Khorana-Innovative Young Biotechnologist Award , 2019, Department of Biotechnology, Govt. of India http://dbtindia.gov.in/sites/default/files/List_final_selected_HGK-IYBA_2019_Awardees_0_0.pdf
Recipient of the SERB Women Excellence Award, 2017, Department of Science and Technology, Government of India
Recipient of the INSA Medal for Young Scientist 2008, Indian National Science Academy, Government of India.
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